This function provides a wrapper to the USEARCH
fastx_findorfs
command line tool to predict ORFs in
a given input fasta file and read the output as data.frame
object.
ORFpred( seq.file, orf.style = 7, min.codons = 200, trans.seqs = FALSE, output = NULL )
seq.file | a fasta file storing the sequences for which open reading frames shall be predicted. |
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orf.style | type of predicting open reading frames (see documentation of USEARCH). |
min.codons | minimum number of codons in the predicted open reading frame. |
trans.seqs | logical value indicating wheter or not predicted open reading frames shall be translated and the corresponding protein sequences stored in the output folder. |
output | path to the folder in which predicted open reading frame sequences shall be stored. |
Robert Edgar. Search and clustering orders of magnitude faster than BLAST. Bioinformatics (2010) 26 (19): 2460-2461.
Hajk-Georg Drost